Effects of Long-term Commercial Extenders for Liquid Storage of Boar Semen

The objective of this study was to compare preservation ability of five long-term commercial semen extenders by means of spermatozoa long-term thermo-resistance survival test. Ejaculates from 21 fertile boars were collected by hand method. Semen gel free volume, motility, viability, sperm concentration, total morphologically abnormal spermatozoa and total number of spermatozoa per ejaculate were determined. The samples of diluted sperm in a semen-dilution rate of 1+4 in Androhep (A), Androstar (AS), Androstar plus (AS), LD and M III were stored at a temperature 17°C up to 96 h. The test was performed on 3 ml samples kept at 38°C in water bath each day and motility of spermatozoa was evaluated at the 1st, 3rd and 5th hour during the incubation. The survival rate significantly decreased parallelly with the storage length in all the tested extenders. The total mean values sperm motility was 39.66 %, 30.77 %, 28.55 %, 28.67 % and 24.50 %, respectively, in A, AS, AS, M III and LD. The total mean value motility observed of Androhep was significantly (P<0.001) higher than of the others extenders. In conclusion, the results of this study showed that Androhep was a better extender than Androstar, M III, Androstar plus and LD in terms of survival rate of boar spermatozoa for long-term liquid preservation.